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WinBreadboard v4.1 is a software-based circuit simulation application for Windows.. This program enables you to create and simulate circuits with the click of a button, and. WBB is advanced circuit simulation software that has the ability to create and simulate printed circuit boards at a. WinBreadboard has a friendly interface, rich feature set and.Acute effects of a capsaicin-rich extract and its main constituents of red pepper on an ex vivo model of intestinal epithelial barrier function.
The aim of the present study was to investigate the potential effects of capsaicin-rich extract of red pepper (CPE) and its main constituents on intestinal epithelial barrier function in vitro. CPE, capsaicin and dihydrocapsaicin (DHC) were added to a model of intestinal epithelial barrier consisting of a Caco-2/HT29-MTX co-culture. Barrier function was studied by measuring transepithelial electrical resistance (TEER). Transepithelial permeability was assessed by measuring the migration of 4 kDa-FITC-Dextran into the incubation medium. Relative expression of ZO-1 and occludin mRNA was analyzed. The effects of CPE, capsaicin and DHC on the expression of ZO-1 and occludin were also investigated by confocal microscopy and Western blot analysis. TEER was significantly reduced after CPE addition. Permeability of the epit
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